Antibody production platform in 96 and 24 deep-well plates, resulting in a daily production throughput of ~1,000 antibodies at 100 -1,500 ug each, suitable for in vitro assays.
Protein Sciences: uHTP Antibody Production
WuXi Biologics uHTP Antibody Production platform can support AI drug development along with automated protein production integrated with expression, purification, and analytical modules. The daily throughput of 1000X transient transfection expression is performed in 96-deep well plates.
96/24 well DWP for 100-1500 μg antibodies; Suitable for in vitro assays
Purification
QC
1-step Purification
A280, SEC-HPLC, Caliper
Automated protein production platform integrated with transfection, purification, and analytical modules
96 Well Plate
3 Weeks
Expression
TAT
Protein with one step purification delivered within 3 weeks. Expression scale is 1~3 mL using 96/24 well plate. Obtain 100~1500 μg protein sufficient for in vitro studies.
• Automated expression and purification • 3 mL culture; average yield ~0.5-1.0 mg/well • One-step Protein A purification
Ultra 96 Service Details: Service Item
Gene synthesis, cloning and plasmid prep all included. Deliverables Duration
QC
96 x 0.8 mL CHO/HEK293 24 x 3 mL CHO/HEK293
100-800 μg
3 weeks
Titer, A280, Caliper (R/NR), and SEC-HPLC
0.5-2 mg
3 weeks
Variation in 96 DWP
The Average Titer and Recovery Rate of “Ultra 96” Platform Left histogram shows the average titer of each kind of molecules expressed in 96 DWP at 1 mL scale. Besides, the average recovery rate after one step purification is above 83%. 1503
Recovery Rate (24DWP)
Average 84%
90%
80%
70%
60%
Titer Variation in 96 DWP
50%
Recovery Rate (24DWP)
1 2 3 4 5 6 7 8 9 101112131415161718192021222324
969
Average 84%
90%
1503
80%
50% 50.0% 55.0% 60.0% 65.0% 70.0% 75.0% 80.0% 85.0% 90.0% 95.0% 1 3 5 7 9 11131517192123252729313335373941434547495153555759 Recovery Rate (96DWP) Average 83% 60% 1 2 3 4 5 6 7 8 9 101112131415161718192021222324 50.0% 55.0% 60.0% 65.0% 70.0% 75.0% 80.0% 85.0% 90.0% 95.0% 1 3 5 7 9 11131517192123252729313335373941434547495153555759 Recovery Rate (96DWP) Average 83%
Need original image 70%
595
1013
969
595
mAb
Bi-specific
Bi-specific Fc fusion
Control
mAb
Fc fusion
Case Study: WuXian TM uHTP Platform
For high throughput antibody production, Ultra 96 can maintain high titer even for some AI designed molecules (853 Abs) and the protein after 1 step purification can be enough for any in vitro assay requirement.
853 Antibodies
50 Antibodies
88 His-VHH
60 Antibodies
4
1.5
1.0
5
1.2
0.8
4
3
3.1 mg
0.6
0.9
3
0.52 mg
1.8 mg
2
0.7 g/L
0.4
2
0.6
1
0.2
1
0.3
0.0
0
0
0
96 Well DWP (1 ml/well)
24 Well DWP (3 ml/well)
Learn more: Protein Sciences: uHTP Antibody Production
About WuXi Biologics
WuXi Biologics is a leading contract research, development and manufacturing organization (CRDMO) that provides end-to-end capabilities to healthcare organizations worldwide. With operations in China, the United States, Ireland, Germany, and Singapore, we enable our partners to effectively and efficiently bring biologics and vaccines to patients worldwide through our comprehensive and high-quality drug development model.
The world’s leading global single-source platform from concept to commercialization.
wuxibiologics.com | info@wuxibiologics.com
3-20-2023
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