Process Cell free E. coli
Timeline 2-3 weeks 2-4 weeks
Soluble protein production Inclusion body and refolding production
Non-Mammalian Platform Features
4-6 weeks
• High-throughput protein refolding condition screen • Screening and engineering of GPCR constructs • Easy to scale-up process for manufacturing • Development of multi-step, non-tag protein purification method
E. coli Platform Features: • Projects start from customer-provided amino acid sequence (with codon optimization), DNA sequence, or plasmids • Deliver product and QC data after multiple purification steps • A toolbox for hard-to-express targets, including host strains, cultural conditions, and plasmid designs • High-throughput screening for evaluation of protein refolding conditions (over 200 refolding conditions established) • Cell-free expression system available for the screening of hard-to-express proteins and various mutants
Process Insect Bac-to-Bac
Timeline 4-6 weeks 3-5 weeks 3-4 weeks 5-6 weeks
flashBac
E. coli Strain Engineering: • Optimizes the purification of desired proteins
S2 transient S2 stable pool
LC/MS results showing before and after purification of a GeneX-deficient protein and elimination of GeneX contamination Case Study: Engineering of E. coli HCP Free Strain
Insect Platform Features: • Projects start from customer-provided amino acid sequence (with codon optimization), DNA sequence, or plasmids • Deliver product and QC data after multiple purification steps • Multiple insect production cell lines available, including: Sf9, Sf21, HighFive, ExpiSf9, Drosophila S2 cell, with flexible transfection strategies (classical Bac-to-Bac, flashBac, S2 transient, and stable pool) • Experience working with challenging molecules like membrane proteins and tag-less proteins requiring, for example, screening and reformation of GPCR mutants or development of multi-step purification processes from scratch • Expertise available for reconstitution of membrane proteins into nanodiscs
BL21
x10 2 1
+ESI Scan (4.848 - 6.214 min. 83 scans) Frag=200
25853.33
0.8 0.6 0.4 0.2
20853.11
36478.08
49085.14
0
20,000
30,000
40,000
50,000
Counts
BL21 GeneX-deficient
x10 4 4 3.5 3 2.5 2 1.5 1 0.5 0
+ESI Scan (5.785 - 6.087 min. 20 scans) Frag=200
25.854.18
10,000
20,000
30,000
40,000
50,000
Counts
Powered by FlippingBook