Transient expression in high-titer CHO and HEK 293 with speed and cost-effectiveness, transitioning seamlessly to CHO stable expression
Protein Sciences: Early-stage R&D Mammalian Platform
Program Features:
WuXi Biologics’ industry-leading research protein generation group produces high-quality proteins and antibodies to meet your schedule and requirements. No project is too large or small. Our team of experts can support the preparation of the most complex proteins or provide one to thousands of standard antibodies and proteins for your diverse needs, including early-stage research, lead candidate screening, immunizations, in vivo studies, biochemical assays, and other research purposes. Projects can be structured by using a FTE or FFS model to meet your project needs. Our labs feature high throughput expression and purification systems to maximize efficiency.
Projects start from customer- provided amino acid sequence (with codon optimization), DNA sequence, or plasmids Deliver high-quality proteins suitable for most in vitro and in vivo studies Provide comprehensive QC data Expi293/HEK293 are default systems for non-antibody reagent proteins (non therapeutics) Utilize our efficient, proprietary, high-yielding WuXia CHO-K1 for antibodies and other therapeutic proteins NS0 are also used
Monoclonal Antibodies
ADC Therapeutics
Bispecific Antibodies
Fc-fusion Proteins
FAB VHH ScFv
Complex mAb formats
Recombinant Proteins
Other Proteins:
• Intracellular • Secreted • Many tag options (his, flag, etc.) • Non tagged • Protein complex
• Antigens • Fc fusions • Cytokines (ILs, TNF, TGFβ, etc.) • Fab & single chain Fv
• ECD of cell surface receptors, TCR • Membrane proteins • His, Flag tag, etc.
Transient Platform
Express Platform
Timeline
Expression
Features
Timeline Expression
Features
3-5 weeks 0.8 mL to 20 L Transient
• Start from codon optimization or customer provided DNA • Deliver product and QC data after 1-2 steps purification
6-7 weeks 20 mL to 100 L Stable Pool
• Start from codon optimization or customer provided DNA • Deliver product and QC data after >2 steps purification
• 4-7 Days • 300 mg/L - 2 g/L • Cell: CHO, HEK293
• mAb/BsAb/recombinant protein • 96DWP - 20 L • mg-g of Protein for Early Discovery
• 4-6 Weeks • Cell: CHO, HEK293, NS0 • 3.5 g/L
• mAb/BsAb/recombinant protein • 20 mL - 100 L • >5g Developability Study and Early CMC Process Development
Gene Synthesis 1-2 weeks
Transient Transfection & Fed-Batch 1 week
Purification and QC 1 week
Gene Synthesis 1-2 weeks
Electrotransfection & Seed Training 2-3 weeks
Fed-Batch 2 weeks
Purification and QC 1 week
High throughput processes (up to 1,000 Ab/day) with up to 3 step automated purification is perfect for large scale screening from AI-based designs. WuXian Transient provides high yield, high quality protein suitable for animal studies and other critical lead molecule assessment projects. WuXian TM Transient Platform
Popular Packages
Reference Yield
Timeline
QC
96 x 0.8 mL 24 x 3 mL
100-800 ug 0.5-2 mg
2 weeks 2-3 weeks 2-3 weeks
A280, Caliper (R/NR), and SEC-HPLC
20 mL
3-10 mg, average 4.5 mg after 2 step purification
<0.5 EU/mg endotoxin control, QC incl. Titer, A280, Caliper (R/NR) or SDS-PAGE, SEC-HPLC
400-1000 mg RP-HPLC, bioburden, DSF, DSC, SPR, LC/MS, SEC-MALS, peptide mapping, glycan profiling, cIEF, CHO HCP, residual DNA, ELISA, Western blot, freeze thaw study, micro-developability testing 3 weeks
1 L Additional QC
WuXian TM Express Platform
If you need more than a few grams of protein (e.g., developability studies), stable pool expression via the WuXian Express Platform is ideal. Our stable pool easily produces high quantity and quality protein in 5-6 weeks in shaker flask, wavebag or bioreactor.
Popular Packages
Reference Yield
Timeline
QC
<0.5 EU/mg endotoxin control, QC incl. Titer, A280, Caliper (R/NR) or SDS-PAGE, SEC-HPLC
20 mL
20 mg
2-3 weeks
1 L - 25 L Additional QC
1 - 25 gram RP-HPLC, bioburden, DSF, DSC, SPR, LC/MS, SEC-MALS, peptide mapping, glycan profiling, cIEF, CHO HCP, residual DNA, ELISA, Western blot, freeze thaw study, micro-developability testing 6-7 weeks
Comparable Product Quality of Different Scales
cIEF
GLYCAN
SEC_LC
SDS_CALIPER
100
100
0 20 40 60 80
0 20 40 60 80
50
50
0
0
Main Peak (%) HMW (%)
LMW (%)
200 L 1st-2000 L 2nd-2000 L Main Peak (%) Acidic Species (%) Basic Species (%)
200 L 1st-2000 L 2nd-2000 L G0 G0F Man5 G1F G2F
NR
R
200 L 1st-2000 L 2nd-2000 L
200 L 1st-2000 L 2nd-2000 L
Similar product quality is observed among different scales of WuXian Express (one transfection)
Learn more: Protein Sciences: Early-stage R&D Mammalian Platform
About WuXi Biologics
WuXi Biologics is a leading contract research, development and manufacturing organization (CRDMO) that provides end-to-end capabilities to healthcare organizations worldwide. With operations in China, the United States, Ireland, Germany, and Singapore, we enable our partners to effectively and efficiently bring biologics and vaccines to patients worldwide through our comprehensive and high-quality drug development model.
The world’s leading global single-source platform from concept to commercialization.
wuxibiologics.com | info@wuxibiologics.com
2-7-2023
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